ELECTROPHYSIOLOGICAL AND BEHAVIORAL PROPERTIES OF 4-AP INDUCED EPILEPTIC ACTIVITY IN MICE
01/29/2020
Flóra Zsófia Fedor1, 2,Zoltán Fekete3, 4, Zsolt Borhegyi5, Gábor Juhász5
1 Doctoral School of Chemical Engineering and Material Sciences, University of Pannonia, Veszprém, Hungary
2 ELTE NAP Neuroimmunology Research Group, Department of Biochemistry, Institute of Biology, Eötvös Loránd University, Budapest, Hungary
3 Research Group for Implantable Microsystems, Faculty of Information Technology & Bionics, Pázmány Péter Catholic University, Budapest, Hungary
4 Centre for Energy Research, Hungarian Academy of Sciences, Budapest, Hungary
5 Department of Biochemistry, Eötvös Loránd University, Budapest, Hungary
4-aminopyridine is a convulsant that can induce epileptoid seizures. The aim of the present study was to investigate the in vivo the effect of intraperitoneally administered 4-aminopyridine in two doses (4 and 10 mg/kg) in mice. EEG, movement (accelerometer) and video recordings were made simultaneously in male B6 mice to specify the details of the seizures. Electrographic seizure escalation started earlier when the higher dose was administered and reached its peak earlier in both doses than behavioral activity. 4-AP in both doses induce an increase in alpha and beta activity bands that is in synchrony with the behavior and at 10mg/kg dose can enhance theta activity. This model is a reliable mouse seizure model that unlike pilocarpine model (Phelan et al., 2015), evokes electrographic seizure faster than behavior and evokes some of the symptoms listed in Racine scale.