CHRONIC PROSTATITIS/CHRONIC PELVIC PAIN SYNDROME IN RATS DECREASES ADULT NEUROGENESIS IN THE HIPPOCAMPUS
Nela Puškaš1, 1, Nikola Šutulović2, Olivera Stanojlović2, Dragan Hrncić2
1 Institute of Histology and Embryology “Aleksandar Đ. Kostić”, School of Medicine, University of Belgrade, Belgrade, Serbia
2 Institute of Medical Physiology “Richard Burian”, School of Medicine, University of Belgrade, Belgrade, Serbia
Chronic Prostatitis/Chronic Pelvic Pain Syndrome (CP/CPPS) is a multifactorial and non-specific clinical syndrome characterized by genitourinary pain, urinary and ejaculatory symptoms in the absence of uropathogenic bacteria. It is frequently accompanied by brain disorders, like depression, anxiety and mood alterations. However, its influence on adult neurogenesis, which can be impaired in above mentioned disorders, has not been addressed. The aim of this study was to investigate influence of CP/CPPS on adult neurogenesis in the dentate gyrus of the hippocampus in rats. For this purpose we induced CP/CPPS in adult rats by intraprostatic injection of 3% λ-carrageenan. Sham operated rats served as controls (0.9% NaCl, Sham). One week after the treatment, animals were sacrified, prostates were sampled for histological evaluation, and brains were carefully removed from the skull and sampled for immunohistochemical (IHC) evaluation with antibody for Ki67, to evaluate proliferative capacity and antibody for doublecortin (DCX), to evaluate number of newly formed neurons. Histological examination showed uniform prostates inflammation with leukocyte infiltration in CP/CPPS and unaltered histology in SHAM rats. Also, analysis of brain samples showed that animals with CP/CPPS showed significantly decreased number of DCX-immunoreactive neurons in dentate gyrus compared with Sham group. Based on our results we can conclude that CP/CPPS has strong influence on adult neurogenesis in the hippocampus in rats.